Increased biosynthesis of secondary metabolites in callus cultures of Hyssopus officinalis L.

Abstract

Currently, callus cultures of medicinal plants are actively used for the production of important biologically active compounds. Investigating conditions that enhance the biosynthesis of these compounds in callus cultures is highly relevant. This article examines the influence of various concentrations of phenylalanine and tyrosine (1—500 µM) on the content of phenolic compounds and the antioxidant activity of three callus cultures of Hyssopus officinalis L. It was found that the addition of phenylalanine at a concentration of 10 µM and tyrosine at a concentration of 100 µM to the nutrient media leads to an increase in the content of phenolic compounds and hydroxycinnamic acids in the callus cultures. An increase in the antioxidant activity of the extracts from the studied callus cultures was recorded at phenylalanine concentrations of 10 and 100 µM, as well as tyrosine concentrations of 1, 10, and 100 µM in the nutrient media.