Cultivation of Chlorella sp. microalgae isolates with biomass productivity assessment

Abstract

Green microalgae belong to a phyletic category of organisms that have adapted to a wide range of ecological conditions. This study explores a novel freshwater microalgae isolate, Chlorella sp. B1, as a potential platform for biomass production. The research evaluated the isolate’s ability to grow in mixotrophic media. The results reveal a relationship between growth rate and dynamic changes in biomass composition. The use of granulated forms of the fertilizer “Fertika Universal” as a nutrient medium significantly increased the optical density of the initial solution to 0.087, more than doubling the results compared to other conventional media and proving advantageous for industrial-scale cultivation. The study recommends using a reduced initial cell concentration of Chlorella sp. B1 in the inoculum, followed by cultivation at 32 °С with enhanced illumination (52 W) and the addition of 0.9 g/L of carbon dioxide for three days in a 500 mL flat-panel photobioreactor.