Повышение биосинтеза вторичных метаболитов в каллусных культурах Hyssopus officinalis L
DOI:
10.5922/vestniknat-2024-3-8
Ключевые слова
лекарственное растение
каллусная культура
рост
биологически активные вещества
аминокислоты
Аннотация
В настоящее время каллусные культуры лекарственных растений активно используются для получения важных биологически активных веществ. Исследование условий, повышающих биосинтез данных соединений в каллусных культурах, является весьма актуальным. В статье изучается влияние различных концентраций фенилаланина и тирозина (1—500 мкМ) на содержание фенольных соединений и антиоксидантную активность трех каллусных культур Hyssopus officinalis L. Было установлено, что добавление в питательные среды фенилаланина в концентрации 10 мкМ и тирозина в концентрации 100 мкМ приводит к повышению содержания фенольных соединений и гидроксикоричных кислот в каллусных культурах. Повышение антиоксидантной активности экстрактов исследуемых каллусных культур было зафиксировано при концентрациях 10 и 100 мкМ фенилаланина, а также при концентрациях 1, 10 и 100 мкМ тирозина в питательных средах.
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33. Musbah H. M., Ibrahim K. M., Ibrahim K. Effects of feeding tyrosine or phenylalanine on the accumulation of polyphenols in Coleus Blumei in Vivo and in Vitro // Journal of Biotechnology Research Center. 2019. Vol. 13, № 1. P. 35—43.
2. Grigore A., Colceru-Mihul S., Bubueanu C. et al. Chemical composition and antioxidant activity of Hyssopus officinalis L. selective fractions obtained by different methods. 2019.
3. Ortiz de Elguea-Culebras G. Sánchez-Vioque R., Berruga M. I. et al. Biocidal potential and chemical composition of industrial essential oils from Hyssopus officinalis, Lavandula× intermedia var. super, and Santolina chamaecyparissus // Chemistry & Biodiversity. 2018. Vol. 15, № 1. P. e1700313.
4. Judžentienė A. Essential Oils in Food Preservation, Flavor and Safety. Vilnius, 2016. Ch. 53. P. 471—479.
5. Tahir M., Khushtar M., Fahad M. et al. Phytochemistry and pharmacological profile of traditionally used medicinal plant Hyssop (Hyssopus officinalis L.) // Applied Pharmaceutical Science. 2018. Vol. 8, № 7. P. 132—140.
6. Zayova E., Geneva M., Stancheva I. et al. Evaluation of the antioxidant potential of in vitro propagated hyssop (Hyssopus officinalis L.) with different plant growth regulators // Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2018. Vol. 10, № 4. P. 295—304.
7. Mobaiyen H., Jafari-Sales A. Antibacterial effects of methanolic extracts of Reum ribes L. and Hyssopus officinalis on some standard pathogenic bacteria // Jorjani Biomedicine Journal. 2019. Vol. 7, № 3. P. 34—44.
8. Гребенникова О. А., Палий А. Е., Хлыпенко Л. А. и др. Биологически активные вещества Hyssopus officinalis L // Орбиталь. 2017. № 1. С. 21—28.
9. Shekarchi M., Hajimehdipoor H., Saeidnia S. et al. Comparative study of rosmarinic acid content in some plants of Labiatae family // Pharmacognosy magazine. 2012. Vol. 8, № 29. P. 37—41.
10. Bernatoniene J., Cizauskaite U., Ivanauskas L. et al. Novel approaches to optimize extraction processes of ursolic, oleanolic and rosmarinic acids from Rosmarinus officinalis leaves // Industrial Crops and Products. 2016. Vol. 84. P. 72—79.
11. Razboršek M. I. Stability studies on trans-rosmarinic acid and GC-MS analysis of its degradation product // Journal of pharmaceutical and biomedical analysis. 2011. Vol. 55, № 5. P. 1010—1016.
12. Efferth T. Biotechnology applications of plant callus cultures // Engineering. 2019. Т. 5, № 1. С. 50—59.
13. Wang S., Cizauskaite U., Ivanauskas L. et al. Effect of elicitors, precursors and metabolicinhibitors on paclitaxel production by Taxus cuspidata cell culture // J. For. Res. 2016. Vol. 27, № 6. Р. 1257— 1263.
14. Veeresham Ciddi V. C., Shuler M. L. Camptothecine from callus cultures of Nothapodytes foetida // Biotechnology Letters. 2000.
15. Martino E., Casamassima G., Castiglione S. et al. Vinca alkaloids and analogues as anti-cancer agents: Looking back, peering ahead // Bioorganic & medicinal chemistry letters. 2018. Vol. 28, № 17. P. 2816—2826.
16. Echeverri J. P., Ortega I. C., Peñuela M. A. et al. Antimicrobial activity of callus and cell suspension cultures extracts of Thevetia peruviana // Revista de la Facultad de Ciencias. 2019. Vol. 8, № 1. P. 45—56.
17. Kayani W. K., Kiani B. H., Dilshad E. et al. Biotechnological approaches for artemisinin production in Artemisia // World Journal of Microbiology and Biotechnology. 2018. Vol. 34. P. 1—14.
18. Benjamin E. D., Ishaku G. A., Peingurta F. A. et al. Callus culture for the production of therapeutic compounds // American Journal of Plant Biology. 2019. Vol. 4, № 4. P. 76—84.
19. Babich O., Sukhik, S., Pungin A. et al. Evaluation of the conditions for the cultivation of callus cultures of Hyssopus officinalis regarding the yield of polyphenolic compounds // Plants. 2021. Vol. 10, № 5. P. 915.
20. Chandran H., Meena M., Barupal T. et al. Plant tissue culture as a perpetual source for production of industrially important bioactive compounds // Biotechnology reports. 2020. Vol. 26. P. e00450.
21. Szoke E., Krajewska A. NMR characterisation of lobeline from Lobelia inflata tissue cultures // Medical Science Monitor. 1998. Vol. 4, № 1. P. BR15—BR19.
22. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures // Physiologia plantarum. 1962. Vol. 15 (3). P. 473—497.
23. Padhi E. M. T., Liu R., Hernandez M. et al. Total polyphenol content, carotenoid, tocopherol and fatty acid composition of commonly consumed Canadian pulses and their contribution to antioxidant activity // Journal of Functional Foods. 2017. Vol. 38. P. 602—611.
24. Štefan M. B., Vuković Rodríguez J., Blažeković B. et al. Total hydroxycinnamic acids assay: Prevalidation and application on Lamiaceae species // Food Analytical Methods. 2014. Vol. 7. P. 326—336.
25. Feduraev P., Skrypnik L., Nebreeva S. et al. Variability of phenolic compound accumulation and antioxidant activity in wild plants of some Rumex species (Polygonaceae) // Antioxidants. 2022. Vol. 11, № 2. P. 311.
26. Skrypnik L., Feduraev P., Golovin A. et al. Biotechnological potential of different organs of mistletoe (Viscum album L.) collected from various host tree species in an urban area // Plants. 2022. Vol. 11, № 20. P. 2686.
27. Demirci T., Çelikkol Akçay U., Göktürk Baydar N. Effects of 24-epibrassinolide and l-phenylalanine on growth and caffeic acid derivative production in hairy root culture of Echinacea purpurea L. Moench // Acta Physiologiae Plantarum. 2020. Vol. 42, № 4. P. 1—11.
28. Masoumian M., Arbakariya A., Syahida A. et al. Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues // African Journal of Biotechnology. 2011. Vol. 10, № 32. P. 6021—6029.
29. Urmantseva V. V., Gaevskaya, O. A., Karyagina, T. B. et al. The effect of amino acids as components of nutrient medium on the accumulation of protoberberine alkaloids in the cell culture of Thalictrum minus // Russian Journal of Plant Physiology. 2005. Vol. 52. P. 388—391.
30. Pavlov A., Ilieva M. The influence of phenylalanine on accumulation of rosmarinic and caffeic acids by Lavandula vera MM cell culture // World Journal of Microbiology and Biotechnology. 1999. Vol. 15. P. 397—399.
31. Sahraroo A., Mirjalili M. H., Corchete P. et al. Enhancement of rosmarinic acid production by Satureja khuzistanica cell suspensions: effects of phenylalanine and sucrose // SABRAO Journal of Breeding & Genetics. 2018. Vol. 50, № 1. P. 25—35.
32. Hakkim F. L., Kalyani S., Essa M. et al. Production of rosmarinic in Ocimum sanctum cell cultures by the influence of sucrose, phenylalanine, yeast extract, and methyl jasmonate // Int J Biol Med Res. 2011. Vol. 2, № 4. P. 1070—1074.
33. Musbah H. M., Ibrahim K. M., Ibrahim K. Effects of feeding tyrosine or phenylalanine on the accumulation of polyphenols in Coleus Blumei in Vivo and in Vitro // Journal of Biotechnology Research Center. 2019. Vol. 13, № 1. P. 35—43.